CD68 antigen expression by human retinal pigment epithelial cells

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CD68 antigen expression by human retinal pigment epithelial cells.

Although a primary role of the retinal pigment epithelium (RPE) is the phagocytosis of aged outer segment membranes, the RPE may also phagocytize particulates via several specific receptors that are characteristically present on mononuclear phagocytes of bone marrow origin. In recent immunophenotypic studies, CD68 monoclonal antibodies (mAb) have been shown to react selectively with a specific ...

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Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells

Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Mate...

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Thrombin-induced VEGF expression in human retinal pigment epithelial cells.

PURPOSE The purpose of the present study was to investigate the effects of thrombin and thrombin in combination with other proangiogenic factors on VEGF expression in hRPE cells. METHODS hRPE cells were stimulated with thrombin TNF-alpha, monocytes, and TGF-beta2. After stimulation, conditioned medium and lysed cells were subjected to ELISA, Western blot analysis, immunocytochemistry, and RT-...

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Bidirectional glutathione transport by cultured human retinal pigment epithelial cells.

PURPOSE To characterize glutathione (GSH) transport by cultured human retinal pigment epithelial (HRPE) cells. METHODS Cultured HRPE cells were pretreated with acivicin for GSH efflux and with buthionine sulfoximine for GSH uptake to prevent the breakdown and resynthesis of GSH. Efflux was measured by the linear rate of accumulation of GSH in the supernatant; uptake was measured using [35S] G...

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ژورنال

عنوان ژورنال: Experimental Eye Research

سال: 1992

ISSN: 0014-4835

DOI: 10.1016/0014-4835(92)90087-9